Journal: Chinese Journal of Cancer Research

Article Title: Tim-3 promotes cell aggressiveness and paclitaxel resistance through NF-κB/STAT3 signalling pathway in breast cancer cells

doi: 10.21147/j.issn.1000-9604.2020.05.02

Figure Lengend Snippet: VEGFC and VEGFR2 protein levels in conditioned media of stable cell lines indicated by ELISA. (A) VEGFC; (B) VEGFR2. VEGF, vascular endothelial growth factor; ELISA, enzyme-linked immunosorbent assay.

Article Snippet: ELISA was performed using the human VEGFC (cat. no. E-EL-H1600; Elabscience) and vascular endothelial growth factor receptor 2 (VEGFR2) ELISA kit (cat. no. E-EL-H1603; Elabscience), according to the manufacturer’s protocol.

Techniques: Stable Transfection, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in immunology

Article Title: Promotion of BST2 expression by the transcription factor IRF6 affects the progression of endometriosis.

doi: 10.3389/fimmu.2023.1115504

Figure Lengend Snippet: FIGURE 1 BST2 levels were overexpressed and positively correlated with the lymphangiogenesis in the endometriosis. (A), BST2 expression levels in the GSE7305 and GSE7307 datasets. (B–D), BST2 mRNA (B) and protein (C, D) expression in endometriosis samples. (E), LYVE1 expression levels in the GSE7305 and GSE7307 datasets. (F), The correlation of BST2 expression and VEGFC expression in the GSE7305 and GSE7307 datasets. (G), The correlation of VEGFC expression and LYVE1 expression in the GSE7305 and GSE7307 datasets. (H), (H, E) staining and Immunohistochemistry (IHC) of BST2, VEGFC and LYVE1 in endometriosis and control group. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: The concentrations of human VEGFC in the cell culture supernatants were measured with a Human VEGFC ELISA Kit (CSB-E04759h, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Expressing, Staining, Immunohistochemistry, Control

Journal: Frontiers in immunology

Article Title: Promotion of BST2 expression by the transcription factor IRF6 affects the progression of endometriosis.

doi: 10.3389/fimmu.2023.1115504

Figure Lengend Snippet: FIGURE 5 Effect of the conditioned medium (CM) of EESCs with BST2 knockdown on HLECs. (A), The Tube formation assay was conducted to check the tube formation ability with the CM of EESCs with BST2 knockdown on HLECs. (B), CCK-8 assay showing the proliferation of HLECs treated with CM from Si-BST2 or Si-NC EESCs for 24h. (C, D), Scratch assay (C) and transwell assay (D) were conducted to assess migration ability of HLECs treated with CM from Si-BST2 or Si-NC EESCs for 24h. (E), The VEGFC protein expression was measured by western blot analysis after transfection with Si-BST2 or Si-NC for 48h. (F), The VEGFC expression from the conditioned medium (CM) of EESCs with BST2 knockdown was detected by ELISA assay. *p < 0.05; **p < 0.01; ****p < 0.0001.

Article Snippet: The concentrations of human VEGFC in the cell culture supernatants were measured with a Human VEGFC ELISA Kit (CSB-E04759h, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Knockdown, Tube Formation Assay, CCK-8 Assay, Wound Healing Assay, Transwell Assay, Migration, Expressing, Western Blot, Transfection, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in immunology

Article Title: Promotion of BST2 expression by the transcription factor IRF6 affects the progression of endometriosis.

doi: 10.3389/fimmu.2023.1115504

Figure Lengend Snippet: FIGURE 8 BST2 promoted migration and lymphangiogenesis of EESCs via the NF-kB signaling pathway. (A, B), The wound healing assays (A) and the transwell assay (B) were performed to measure the cell migration ability of EESCs after transfection with Si-BST2 and/or IL-1b. (C), The western blot analysis was used to examine the migration-related protein after transfection with Si-BST2 and/or IL-1b. (D, E), The tube formation assay was conducted to check the tube formation ability with the CM of EESCs with Si-BST2 and/or IL-1b on HLECs. (F), The western blot analysis was used to measure the VEGFC after transfection with Si-BST2 and/or IL-1b. *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: The concentrations of human VEGFC in the cell culture supernatants were measured with a Human VEGFC ELISA Kit (CSB-E04759h, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Migration, Transwell Assay, Transfection, Western Blot, Tube Formation Assay

Journal: Frontiers in immunology

Article Title: Promotion of BST2 expression by the transcription factor IRF6 affects the progression of endometriosis.

doi: 10.3389/fimmu.2023.1115504

Figure Lengend Snippet: FIGURE 11 BST2 partially rescued the effects of IRF6 on the migration and lymphangiogenesis in EESCs. (A, B), Wound healing assay (A) and Transwell assay (B) detected the cell migration ability after transfection with Oe-IRF6 and/or Si-BST2 for 48h. (C), The western blot analysis examined the migration- related proteins after transfection with Oe-IRF6 and/or Si-BST2 for 48h. (D), The tube formation assay checked the tube formation ability with the CM of EESCs transfected with Oe-IRF6 and/or Si-BST2 on HLECs. (E), The western blot analysis measured the VEGFC after transfection with Oe-IRF6 and/or Si-BST2. *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: The concentrations of human VEGFC in the cell culture supernatants were measured with a Human VEGFC ELISA Kit (CSB-E04759h, CUSABIO, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Migration, Wound Healing Assay, Transwell Assay, Transfection, Western Blot, Tube Formation Assay

Journal: Oncology Research

Article Title: Exosomal miR-224-3p promotes lymphangiogenesis and lymph node metastasis by targeting GSK3B in gastric cancer

doi: 10.32604/or.2024.050431

Figure Lengend Snippet: Exosomal miR-224 was screened to be associated with LNM of GC (A) Volcano plot analysis of serum sequencing differential miRNAs (LNM vs . nonLNM). (B) Venn diagram of the serum sequencing differential dataset vs . the GEO dataset. (C) Relative expression of serum miR-224-3p in HC and GC in GSE112264 datasets. (D) Relative expression of serum miR-224-3p in nonLNM and LNM. (E) Correlation analysis between the relative expression of serum miR-224-3p and serum VEGFC. (F) Relative expression of miR-224-3p in serum EVs and exo-free serum. (G) Schematic diagram of LVD in tumor tissues. (H) Correlation analysis between the relative expression of serum miR-224-3p and LVD in tumor tissues. * p -value < 0.05, ** p -value < 0.01, *** p -value < 0.001.

Article Snippet: The remaining steps of serum vascular endothelial growth Factor C (VEGFC) detection were performed according to the ELISA kit protocol (Elabscience, Wuhan, China).

Techniques: Sequencing, Expressing