Journal: Chinese Journal of Cancer Research

Article Title: Tim-3 promotes cell aggressiveness and paclitaxel resistance through NF-κB/STAT3 signalling pathway in breast cancer cells

doi: 10.21147/j.issn.1000-9604.2020.05.02

Figure Lengend Snippet: VEGFC and VEGFR2 protein levels in conditioned media of stable cell lines indicated by ELISA. (A) VEGFC; (B) VEGFR2. VEGF, vascular endothelial growth factor; ELISA, enzyme-linked immunosorbent assay.

Article Snippet: ELISA was performed using the human VEGFC (cat. no. E-EL-H1600; Elabscience) and vascular endothelial growth factor receptor 2 (VEGFR2) ELISA kit (cat. no. E-EL-H1603; Elabscience), according to the manufacturer’s protocol.

Techniques: Stable Transfection, Enzyme-linked Immunosorbent Assay

Journal: Oncology Research

Article Title: Exosomal miR-224-3p promotes lymphangiogenesis and lymph node metastasis by targeting GSK3B in gastric cancer

doi: 10.32604/or.2024.050431

Figure Lengend Snippet: Exosomal miR-224 was screened to be associated with LNM of GC (A) Volcano plot analysis of serum sequencing differential miRNAs (LNM vs . nonLNM). (B) Venn diagram of the serum sequencing differential dataset vs . the GEO dataset. (C) Relative expression of serum miR-224-3p in HC and GC in GSE112264 datasets. (D) Relative expression of serum miR-224-3p in nonLNM and LNM. (E) Correlation analysis between the relative expression of serum miR-224-3p and serum VEGFC. (F) Relative expression of miR-224-3p in serum EVs and exo-free serum. (G) Schematic diagram of LVD in tumor tissues. (H) Correlation analysis between the relative expression of serum miR-224-3p and LVD in tumor tissues. * p -value < 0.05, ** p -value < 0.01, *** p -value < 0.001.

Article Snippet: The remaining steps of serum vascular endothelial growth Factor C (VEGFC) detection were performed according to the ELISA kit protocol (Elabscience, Wuhan, China).

Techniques: Sequencing, Expressing

Journal: OncoImmunology

Article Title: Adenosine A2a receptor promotes lymphangiogenesis and lymph node metastasis

doi: 10.1080/2162402x.2019.1601481

Figure Lengend Snippet: Figure 7. VEGF-C levels are reduced in A2a-deficient sentinel lymph nodes. WT and A2a-deficient mice were injected with 2.5 × 105 B16-CD73+ melanoma cells in the foot pad and two weeks later tumor-draining lymph nodes and contralateral lymph nodes were collected. Primary tumors and lymph node were homogenized, centrifuged and supernatants were assayed for VEGF levels by ELISA. (a) Fold-increase in VEGF-A, C and D in tumor-draining lymph nodes (TdLN) over matched contraleral lymph nodes (cLN). (b) Levels of VEGF-A, C and D in tumors. Means ± standard errors are shown. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 using a Student t test. Results of 2 independent experiments with 10 mice per group were pooled.

Article Snippet: VEGF-C ELISA kit was purchased from Cusabio.

Techniques: Injection, Enzyme-linked Immunosorbent Assay

Journal: OncoImmunology

Article Title: Adenosine A2a receptor promotes lymphangiogenesis and lymph node metastasis

doi: 10.1080/2162402x.2019.1601481

Figure Lengend Snippet: Figure 8. The number of VEGF-C secreting B cells is reduced in A2a-deficient sentinel lymph nodes. WT and A2a-deficient mice were injected with 2.5 × 105 B16-CD73+ melanoma cells in the foot pad. (a) Two weeks later, tumor-draining (TdLN) and contralateral (cLN) lymph nodes were collected and assayed for VEGF-C levels by ELISA and for flow cytometry for (b) CD45.2+/CD19+ B cells, and (c) CD45.2+/CD11b+/F4/80+

Article Snippet: VEGF-C ELISA kit was purchased from Cusabio.

Techniques: Injection, Enzyme-linked Immunosorbent Assay, Flow Cytometry